Skip to main content

Service

The Core Facility offers expertise and technical assistance for your project, particularly in the following applications:

(for any applications not listed below, please inquire)

Differential Gene Expression

  • 3′ mRNA 
    • Standard [ >100 ng total RNA Input]
    • Low Input [ 10-100 ng total RNA Input ]

    • FFPE [FFPE-derived, or otherwise very degraded (DV200>2%) total RNA]

  • Total RNA
  • Small RNA
  • tRNA

Single-cell sequencing (10x Genomics)

  • scRNA seq
  • scRNA seq –Flex
  • sc TCR or BCR seq
  • sc Feature seq
  • sc ATAC seq
  • sc MultiOmics (combinations of the above)
  • single nuclei RNA seq
  • single nuclei RNA seq -FFPE

Spatial transcriptomics (10x Genomics)

  • Visium spatial transcriptomics – FFPE
  • Xenium spatial transcriptomics – FFPE

Cut&Run

High resolution genome-wide profiles of histone posttranslational modifications (PTMs) and chromatin-associated proteins (e.g. TFs and chromatin remodelers)

Proteomics

Olink(R) Reveal for assaying >1000 proteins in serum or plasma samples (with sequencing read-out)

Quality Control

  • Measurement of concentration (Qubit – QFX Biozym)
  • Integrity analysis (Bioanalyzer, Fragment Analyzer)

Access to Q-RT-PCR workflow

QuantStudio 5 + EpMotion liquid handling for preparing 384w q-RT-PCR plates

Grant Proposal Assistance

  • Development of project design
  • Cost calculation (offers)
  • Letter of support